Mucinous adenocarcinoma of salivary gland: a case report / 华西口腔医学杂志

Mucinous adenocarcinoma is a rare epithelial malignant tumor which usually arises in appendix, pancreas, breast and other sites, rarely occurs in salivary gland. In this article, a mucinous adenocarcinoma of salivary gland was reported and relevant literatures were reviewed.

Siblings present different clinical manifestations in white sponge nevus / 华西口腔医学杂志

White sponge nevus (WSN) is a rare, autosomal-dominant hereditary disease. This article reported two siblings affected by white sponge nevus. Because of smoking or not, they presented different clinical manifestations.

The expression of S-100 protein, CD1a, CD83 and Ki-67 in oral Langerhans cell histiocytosis / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study clinicopathological features, diagnosis, differential diagnosis of oral Langerhans cell histiocytosis (LCH), retrospective clinicopathologic study was carried on and a variety of immune phenotype were detected.</p><p><b>METHODS</b>The clinicopathological features of 29 cases of oral LCH were analyzed. The immunohistochemical staining of S-100 protein, CD1a, CD83 and Ki-67 were used in above cases by immunohistochemical streptavidin-biotin peroxidase (SP) and Elivison two-step method. Statistical analysis was adopted for the results.</p><p><b>RESULTS</b>Of the 29 cases of LCH, the expression of S-100 protein and CD1a were positive in 24 cases and negative in 5 cases, so 5 cases were excluded from the diagnosis of LCH. Among 24 cases of LCH, 15 patients were male and 9 were female. The median age was 7.50 years. 14 lesions were in the mandible, 5 were in the maxilla and 5 involved the mandible and maxilla. 9 cases were in stage I, 13 in stage II and 2 in stage III, according to Bartnick classification. Immunohistochemistry showed all 24 cases staining for S-100 protein and CD1a were positive. Comparing with maxillofacial lesions involved soft tissue, Ki-67 positive rate was lower and CD83 positive rate was higher in maxillofacial single bone lesion.</p><p><b>CONCLUSION</b>The immunohistochemical staining of S-100 protein and CD1a are important for the diagnosis of LCH. Maxillofacial bone single LCH might have lower proliferative activity and a higher state of maturity. Maxillofacial LCH involved soft tissue might have a higher proliferative activity and a lower state of maturity.</p>

Immunohistochemical study of sialyl Lewis(a) antigen in oral squamous cell carcinoma: the association of sialyl Lewis(a) expression with local lymph metastasis / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to screen sialyl Lewis(a) (sLe(a)) in the tumors of patients with oral squamous cell carcinoma (OSCC) and to explore the association of sialyl Lewis(a) expression with local lymph node metastasis.</p><p><b>METHODS</b>Specimen from 38 patients with primary OSCC were obtained and analyzed by immunohistochemical methods.</p><p><b>RESULTS</b>The expression of sLe(a) protein, but not E-selectin, of OSCCs significantly correlated to the local lymph node metastasis. sLe(a) was expressed in 79% (15/19) of the metastatic cases compared with 21% (4/19) of the non-metastasis ones, indicated the association of sLe(a) expression with the local lymph involvement.</p><p><b>CONCLUSION</b>High expression of sLe(a) in OSCC may be related to the metastasis of cervical lymph nodes and it seems useful in predicting poor prognosis in OSCC.</p>

A clinical and pathological study on primary oral mucosal malignant melanoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to investigate the association between clinical and pathological characteristics of primary oral mucosal malignant melanoma and the prognosis of this disease.</p><p><b>METHODS</b>Clinical and pathological characters of 73 cases primary oral mucosal malignant melanoma were investigated. The association between risk factors, such as black macule, lymph node metastases, invasive depth, clinic stages, pathologic types, and prognosis was analyzed using Kaplan-Meier survival analysis and Log rank test.</p><p><b>RESULTS</b>The age of the patients ranged from 24 to 80 years (Median age, 50). Among the patients, 43 were males and 30 were females. The most common locations of the tumor were palate and gingiva. The clinic stages of these patients were as the following: Stage I (46/73), Stage II (24/73), Stage III (3/73). The most common pathological type was nodular (44/73), followed by lentiginose malignant melanoma (15/73) and superficial spreading (1/73). According to the configuration of tumor cells, the most common type was the mixed cell type (37/73), followed by the epitheloid cell type (27/73) and the spindle cell type (9/73). Among the 73 patients, 43 were followed up, the 3-year and 5-year survival rates were 19.86% and 11.91% respectively. Black macule, lymph node metastases, invasive depth, clinic stages, pathologic types and therapeutic methods were significantly associated with the prognosis (P < 0.05).</p><p><b>CONCLUSION</b>The prognosis of the primary oral mucosal malignant melanoma is associated with black macule, lymph node metastases, invasive depth, clinic stages, pathologic types, and therapeutic methods.</p>

Flowcytometry DNA analysis of oral and maxillofacial non-Hodgkin's lymphoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to investigate the relationship between the results of flowcytometry analyses of different clinical stage, location, pathologic grade and cell origin of oral and maxillofacial non-Hodgkin's lymphoma (NHL), and the diagnostic value of flowcytometry analysis in lymphoma.</p><p><b>METHOD</b>This study analyzed 50 oral and maxillofacial NHL cases and 10 reactive lymph nodes (formalin fixed and paraffin embedded) by flowcytometry (FCM).</p><p><b>RESULTS</b>Reactive lymph nodes were all diploid. The diploid rate of NHL was 54%, and aneuploidy rate was 46%. There was statistically significant difference between reactive lymph nodes and NHL in the DNA ploidy status and cell cycle data (SPF, CV, S + G2/M, DI). The S phase fraction (SPF) and S + G2/M had close relationship with the grade of NHL. SPF value and DNA ploidy status had no obvious relationship with the prognosis.</p><p><b>CONCLUSION</b>The results suggested that the FCM had diagnostic value in NHL, especially when the morphological diagnosis was difficult. Although the cell cycle data had no prognostic value, SPF and SPF + G2/M can show the proliferative status of NHL, which can help clinical doctor select therapeutic method.</p>

THE PROTECTIVE EFFECTS OF SOYBEAN ISOFLAVONE ON GENETIC TOXICITY INDUCED BY DI-N-BUTUL PHTHALATE IN MICE / 营养学报

Objective To investigate the protective effects of soybean isoflavone(SI) on genetic toxicity induced by di-n-butul phthalate(DBP) in mice.Method(1) Micronucleus test:40 male 7 w old Kunming mice were randomized into 4 groups:High and low dose SI intervention groups,DBP model group,and solvent control group.SI intervention groups were given different doses of SI(50,100mg/kg) for 30 d,meanwhile,the DBP group and solvent group were given 0.5% sodium carboxymethyl cellulose.Then all groups were treated by 0.5g/kg DBP for 5d except solvent group.Mice were sacrificed 6 hour after last treatment,and then counting micronucleated cells in bone marrow.(2) Sperm malformation test:40 male 6w old Kunming mice were grouped and treated the same as micronucleus test.Mice were sacrificed at 35 day after the first treatment,and then sperm quantity,motility,viability and abnormality rate were calculated.Result Micronucleus rate and sperm abnormality rate of SI intervention group were lower than DBP model group,while sperm motility and viability were higher than DBP model group.Conclusion SI can relieve the genetic toxicity induced by DBP in mice.